METHOD OF ISOLATING DOUBLE-STRANDED DNA CONTAINING SIMPLE SEQUENCE REPEATS

摘要

It is intended to provide a method for isolating a DNA containing simple sequence repeats (SSRs). Namely, a method of isolating a double-stranded DNA containing SSRs which comprises (1) the step of incubating a double-stranded DNA plasmid library together with a probe containing the simple sequence repeats to be isolated in the presence of a homologous recombinant protein and (2) the step of isolating a plasmid hybridized with the probe. A kit and a probe which are useful in this method and a method of producing the probe are also provided. This probe is composed of a region comprising the simple sequence repeats and base sequence(s)other than the simple sequence repeats which are attached to one of the 5'-side and the 3'-side thereof or both of these sides. This probe may be modified in the region attached to the 3'-side with a bonding ligand such as biotin.