METHOD FOR PRODUCING INSULIN FROM A NATURAL SOURCE AND INSULIN

摘要

The inventive method for producing insulin consists in using a reindeer pancreas which is homogenized in an ethanol hydrochloride solution, whereupon extraction is effected followed by clarifying the solution to obtain a supernatant fluid which is subjected to an ion-exchange chromatography and isoelectric precipitation to produce insulin as the desired product which is purified by a high-efficiency reversed-phase liquid co-chromatography. The resultant insulin is capable of competing for a bond with an insulin receptor at a concentration over 100 ng/ml.