发明名称 |
Methods for transforming immature maize embryos |
摘要 |
Methods are provided for transforming freshly isolated, immature maize embryos and for producing transgenic maize plants. The methods comprise obtaining immature embryos from a maize plant, contacting the embryos with an auxin-depleted or phytohormone-depleted transformation support medium and introducing a nucleotide construct into cells from the embryos prior to subjecting the embryos to conditions which promote embryogenic-tissue formation. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.
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申请公布号 |
US7057089(B2) |
申请公布日期 |
2006.06.06 |
申请号 |
US20010993080 |
申请日期 |
2001.11.13 |
申请人 |
PIONEER HI-BRED INTERNATIONAL, INC. |
发明人 |
RANCH JEROME P.;MARSH WALLACE A. |
分类号 |
C12N15/05;A01H4/00;C12N15/82;C12N15/90 |
主分类号 |
C12N15/05 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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