发明名称 |
ENZYMATIC REACTIONS IN THE PRESENCE OF KETO ACIDS |
摘要 |
Conversion in vitro of X-Gly to X-alpha-hydroxy-Gly or X-NH2 (X being a peptide or any other compound having a carbonyl group capable of forming a covalent bond with glycine) is accomplished enzymatically in the presence of keto acids, or salts or esters thereof, to provide a good yield without the necessity of catalase or similar enzymatic reaction enhancers. Peptidylglycine α-amidating monooxygenase (PAM) is a preferred enzyme for catalyzing the conversion. Alternatively, peptidylglycine α-hydroxylating monooxygenase (PHM) is utilized to convert X-Gly to X-alpha-hydroxy-Gly which may be recovered, or optionally may be simultaneously or sequentially converted to an amide by either a Lewis base or action of the enzyme peptidyl α-hydroxyglycine α-amidating lyase (PAL). Both PHM and PAL are functional domains of PAM. |
申请公布号 |
CA2586128(A1) |
申请公布日期 |
2006.06.01 |
申请号 |
CA20052586128 |
申请日期 |
2005.11.23 |
申请人 |
UNIGENE LABORATORIES INC. |
发明人 |
CONSALVO, ANGELO P.;MEHTA, NOZER M.;STERN, WILLIAM;GILLIGAN, JAMES P. |
分类号 |
C12P13/02;C12P21/06 |
主分类号 |
C12P13/02 |
代理机构 |
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代理人 |
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地址 |
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