发明名称 |
CATTLE BETA-CASEIN GENE TARGETING VECTOR USING HOMOLOGOUS RECOMBINATION |
摘要 |
<p>The present invention relates to a bovine beta-casein gene targeting vector comprising (1) a first region having a length of 5 to 12 kb which is homologous to the promoter and its flanking nucleic acid sequences of bovine beta-casein gene, and comprising exon 1, intron 1, and exon 2 of bovine beta-casein gene; (2) a region for cloning a nucleic acid coding for desired proteins; (3) a region for coding a positive selection marker; (4) a second region having a length of 2.8 to 3.5 kb which is homologous to the nucleic acid sequences of bovine beta-casein gene, and comprising exon 5, 6, 7 and 8, and intron 5, 6 and 7 of bovine beta-casein gene; wherein the nucleic acid segment corresponding to the first region is located upstream to the nucleic acid segment corresponding to the second region in the 5' -3' arrangement of beta-casein gene. The present invention also relates to method producing the transgenic cattle which is bovine beta-casein gene-targeted with a gene coding a desired protein using the said vector and obtaining a large scale of a desired protein from the milk of the said transgenic cattle.</p> |
申请公布号 |
WO2006057499(A1) |
申请公布日期 |
2006.06.01 |
申请号 |
WO2005KR03923 |
申请日期 |
2005.11.18 |
申请人 |
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY;HAN, YONG-MAHN;LEE, KYUNG-KWANG;CHANG, MIRA;KOO, DEOG-BON |
发明人 |
HAN, YONG-MAHN;LEE, KYUNG-KWANG;CHANG, MIRA;KOO, DEOG-BON |
分类号 |
C12N15/85;A01H5/00;C07H21/02;C12N15/00;C12N15/63;C12N15/877 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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