发明名称 |
METHOD OF PREPARING A PROLIFERATION-REGULATED RECOMBINANT ADENOVIRAL VECTOR, KIT AND VECTOR |
摘要 |
Provided is a method of preparing a proliferation-regulated recombinant adenoviral vector effectively, comprising preparing a proliferation-regulated vector plasmid by preparing a restriction enzyme-recognizing sequence in a vector plasmid having a proliferation-regulating unit and having an E1A region, a protein-coding region in a E1B region, a poly(A) signal sequence, and a recombinase-recognizing sequence in that order from upstream, by deleting an endogenous promoter in the E1A region or an endogenous promoter regulating expression of the protein-coding gene in one protein-coding region of the E1B region thereof and inserting the restriction enzyme-recognizing sequences in the deficient site, and introducing a promoter expressing specifically in a target organ in the restriction enzyme-recognizing sequence in the restriction enzyme-recognizing sequence; and additionally, integrating the proliferation-regulated vector plasmid into a vector plasmid having a adenoviral genome prepared by deleting the E1 region.
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申请公布号 |
EP1662004(A1) |
申请公布日期 |
2006.05.31 |
申请号 |
EP20040748157 |
申请日期 |
2004.07.26 |
申请人 |
NAGOYA INDUSTRIAL SCIENCE RESEARCH INSTITUTE |
发明人 |
KOSAI, KENICHIRO;NAGANO, SATOSHI |
分类号 |
C12N15/09;C12N15/861;A61K35/76;A61K48/00;A61P35/00;C07K14/075;C12N15/34;(IPC1-7):C12N15/861 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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