发明名称 |
Serotype and dengue group specific flurogenic probe based PCR (TaqMan) assays against the respective C and NS5 genomic and 3' non-coding regions of dengue virus |
摘要 |
Five fluorogenic probe hydrolysis reverse transcriptase-polymerase chain reaction (RT-PCR) (TaqMan(TM)) assays are described for serotype-specific detection of dengue 1-4 and group-specific detection of dengue viruses. Type- and group-specific oligonucleotide primers and fluorogenic probes were designed against conserved regions of the dengue genome. The invention provides TaqMan PCR assays, which are rapid, sensitive, and specific screening and serotyping tools for the epidemiological study of dengue infections.
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申请公布号 |
US7052878(B1) |
申请公布日期 |
2006.05.30 |
申请号 |
US20040952584 |
申请日期 |
2004.09.29 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY |
发明人 |
CALLAHAN JOHNNY DALE;TEMENAK JOSEPH JOHN |
分类号 |
C12P19/34;C12Q1/70 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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