发明名称 |
Alteration of restriction endonuclease specificity by genetic selection |
摘要 |
Methods and compositions are provided for altering the DNA recognition and cleavage characteristics of an endonuclease without prior knowledge of the endonuclease's three-dimensional structure and/or amino acid residues responsible for activity and/or specificity. Methods include subjecting a mutagenized endonuclease gene library to a genetic selection in prokaryotic cells which tolerate the expression of mutated endonuclease and where the endonuclease is active and determining the altered recognition-site specificity for the endonuclease.
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申请公布号 |
US7052897(B2) |
申请公布日期 |
2006.05.30 |
申请号 |
US20040501196 |
申请日期 |
2004.07.09 |
申请人 |
NEW ENGLAND BIOLABS |
发明人 |
SAMUELSON JAMES C.;XU SHUANG-YONG |
分类号 |
C12N9/22;C12N9/10;C12N9/16;C12N15/10;C12N15/85;C12Q1/68 |
主分类号 |
C12N9/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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