摘要 |
1,221,272. Cytolytic enzyme. KYOWA HAKKO KOGYO CO. Ltd. 3 June, 1969 [28 June, 1968], No. 27960/69. Heading C3H. A cytolytic enzyme is produced by culturing a micro-organism belonging to the genus Coprinus which is capable of producing the enzyme in a nutrient medium, and recovering the enzyme from the resultant culture liquor. The enzyme may be isolated by precipitation with ammonium sulphate followed by purification by dialysis. The culture medium may contain yeast extract, dried yeast, rice bran, indole-acetic acid, 2,4-dichlorophenoxy acetic acid, naphthalene acetic acid, gibberellin or kinetin, the other ingredients being conventional. The organism used may be Coprinus macrorhizus var. microsporus. Coprinus radians or Coprinus micaceus. |