METHOD FOR MEASURING HEPTOGLOBIN LEVEL IN BLOOD SERUM AND KIT THEREFOR

摘要

The present invention relates to a method for measuring heptoglobin level in blood serum by using antibodies for a and ß subunits of heptoglobin, and a kit for immunodetection of heptoglobin which comprises the antibodies for a and ß subunits of heptoglobin for measuring heptoglobin level in blood serum. A method for measuring heptoglobin level in blood serum of the present invention comprises the steps of: reacting blood serum of a subject with an antibody(ah antibody) which binds specifically to a subunit of a dimeric heptoglobin and an antibody(ßh antibody) which binds specifically to ß subunit of a dimeric heptoglobin; and, measuring level of proteins which react with the ah antibody and ßh antibody in blood serum and have a molecular weight of more than 100kDa. The method for measuring heptoglobin level in blood serum can be practically applied for the early diagnosis of dieases which disrupt erythrocytes, since only the level of normal heptoglobin except for monomelic and degraded hep- toglobins can be specifically measured by the said method.