METHOD FOR PRODUCTION OF A HETEROLOGOUS PROTEIN USING YEAST-TYPE HOST CELLS

摘要

A heterologous protein is produced by means of a method in which yeast-type host cells are prepared, which, in addition to the at least one endogenous homologous DNA sequence coding for a first calnexin, comprise at least one recombinant DNA sequence coding for the protein and at least one additional recombinant DNA sequence coding for at least one second calnexin. The protein in the host cell represents a heterologous secretable protein. The host cells are induced to express the heterologous protein by means of the at least one sequence coding for the protein and to overexpress calnexins by means of the at least one additional sequence coding for the at least one second calnexin. The host cells are then induced to secrete the heterologous protein. The secreted heterologous protein is then separated off.