Methods and materials relating to mycoplasma polypeptide preparations having the ability to increase calcium release from porcine ciliated tracheal cells

摘要

A substantially pure mycoplasma polypeptide, with a molecular weight between about 30 kDa and 150 kDa, increases calcium release from porcine ciliated tracheal cells. The polypeptide can be used to generate polypeptide fragments that have the ability to block mycoplasma-induced calcium release and can also be used to generate antibodies that bind to the mycoplasma polypeptide. Also disclosed are methods for identifying inhibitors of mycoplasma-induced calcium released from porcine ciliated tracheal cells.