FLUORESCENCE-BASED KINETIC DETERMINATION OF TRANSGLUTAMINASE ACTIVITY

摘要

The invention relates to a method for the fluorescence-based kinetic measurement of transglutaminase activity. Said method is characterized in that the transglutaminase is reacted with a modified peptide substrate in which a fluorophore is bonded directly to the N terminal of the peptide and a glutaminic acid radical carries a fluorescence quencher in position 5, said fluorescence quencher being bonded to position 5 via a spacer containing an amine function so as to form an amide bond, such that the fluorescence quencher is separated as a result of the transglutaminase activity, and the resulting fluorescence of the remaining molecule is measured.