METHOD OF DETECTING ANTIBODY WITH THE USE OF CELL-FREE PROTEIN SYNTHESIS SYSTEM AND METHOD OF SCREENING SPECIFIC PROTEIN

摘要

It is intended to provide a system whereby an antibody in a specimen can be surely and quickly detected. To achieve this object, various attempts have been made to examine whether or not the cell-free protein synthesis method is applicable to this assay system. As an important factor for achieving the above object, various attempts have been made to improve the expression manner of a specific protein. As a result, it is found out that a target antibody in a specimen can be assayed by preparing a specific protein as a fused protein by the cell-free protein synthesis method and contacting this unpurified fused protein with the specimen.