发明名称 |
Methods for perfusion and plating of primary hepatocytes and a medium therefore |
摘要 |
The present invention provides methods for culturing primary hepatocytes with improved long term function and improved viability, by plating the hepatocytes in the presence of an anti-oxidants) as well as an agent(s) which is a functional inhibitor of enzymes that generate reactive oxygen and reactive nitrogen species. One preferred embodiment provides a combination of 2-oxo-thizolidine and tocopherol succinate. Another preferred embodiment provides a combination of N<SUP>G</SUP>-methylarginine and mannitol.
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申请公布号 |
US2006110826(A1) |
申请公布日期 |
2006.05.25 |
申请号 |
US20050521920 |
申请日期 |
2005.07.11 |
申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
发明人 |
LEACH JOHN K.;TANNENBAUM STEVEN R. |
分类号 |
C12N5/00;C12N5/071;G01N33/50 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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