| 发明名称 |
METHOD OF PREPARING POLYPEPTIDES IN CELL-FREE SYSTEM AND DEVICE FOR ITS REALIZATION |
| 摘要 |
<p>The present invention provides a method for synthesis polypeptides in a cell-free system by which products of synthesis are branched in a low molecular weight fraction and a fraction which contains high molecular weight components with the target polypeptide, the main part of the low molecular weight fraction is removed via at least one part of the second porous barrier, the ratio of the volume of the fractions of feed solution and expendable components to the volume of the fraction containing the target polypeptide is chosen, modes of supply of the feed solution and expendable components of the fraction are realized.</p> |
| 申请公布号 |
EP1068348(B1) |
申请公布日期 |
2006.05.24 |
| 申请号 |
EP19990917898 |
申请日期 |
1999.03.27 |
| 申请人 |
ROCHE DIAGNOSTICS GMBH;INSTITUTE OF PROTEIN RESEARCH |
发明人 |
BIRYUKOV, SERGEY VLADIMIROVICH;SIMONENKO, PETER NIKOLAYEVICH;SHIROKOV, VLADIMIR ANATOLIEVICH;MAJOROV, SERGEY GENNADIEVICH;SPIRIN, ALEXANDER SERGEYEVICH |
| 分类号 |
C12P21/02;G01N33/68;C12M1/00;C12M1/40;C12N15/00;C12P21/00;C12Q1/68 |
主分类号 |
C12P21/02 |
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