发明名称 |
ein verfahren zur herstellung multispezifischer antikörper die heteromultimere und gemeinsame komponenten besitzen |
摘要 |
The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method further involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and/or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide. The method allows for the enhanced formation of the desired heteromultimer relative to undesired heteromultimers and homomultimers. |
申请公布号 |
DE69830901(T2) |
申请公布日期 |
2006.05.24 |
申请号 |
DE1998630901T |
申请日期 |
1998.04.30 |
申请人 |
GENENTECH INC., SAN FRANCISCO |
发明人 |
ARATHOON, ROBERT;CARTER, J.;MERCHANT, M.;PRESTA, G. |
分类号 |
C12N15/09;C12N15/13;A61K38/00;C07K16/00;C07K16/28;C07K16/32;C07K16/46;C12N5/10;C12P21/08 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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