Interferon-ß protein analogs in which the asparagine at position 25, numbered in accordance with native interferon-ß, is deamidated exhibit a biological activity of native human interferon-ß at an increased level and do not require HA for protein stabilization. The deamidated product is suitable for large scale manufacturing for incorporation in HA-containing or HA-free therapeutics for treatment of diseases including multiple sclerosis. An endoproteinase-C peptide map technique that produces a fingerprint profile for proteins using an enzymatic digest followed by RP-HPLC is also useful in quality control as an ID and/or quantitative test for the deamidated products.
申请公布号
WO2006053134(A2)
申请公布日期
2006.05.18
申请号
WO2005US40758
申请日期
2005.11.10
申请人
CHIRON CORPORATION;FURUYA, KENJI;JOHNSON-JACKSON, DEBORAH;RUSCIO, DIANA, TIERRA
发明人
FURUYA, KENJI;JOHNSON-JACKSON, DEBORAH;RUSCIO, DIANA, TIERRA