Use of betel leaf extract to induce IFN-gamma production from human peripheral blood T cells and as a Th1 type immunomodulator

摘要

This invention relates to use of betel leaf extract to induce IFNgamma from human peripheral blood mononuclear cells and as a Th₁ type immuno modulator, wherein the said method comprises the steps of, preparing water extract of betel leaf, preparation of human peripheral blood mononuclear cells, incubation of hPBMC with betel leaf extract for a period of 18-48 hours, extraction of RNA for cytokine specific RT-PCR or for flow cytometry for the detection of intracellular cytokine protein, subjecting RNA for RT-PCR to obtain PCR products using IFNgamma specific known primers, and enhancement of IFNgamma as reflected by IFNgamma specific band.