PROCESS FOR OBTAINING DRY STEROIDS DERIVATIVE FROM ERGOSTANE

摘要

The present invention refers to a process to obtain ergostane derivative dry steroids, such as physalins, including steps of: (a) grinding of dried parts of plant (s) from the Solanaceae species; (b) treatment of the said plant (s)' parts from step (a) with sodium chloride saline solution (physiological buffer) for a proper period of time; (c) filtration of the resulting solute of step (b) and, then, its partition in methylene chloride or solvent which is unsolvable in water, such as chlorophorm or ethyl acetate; (d) washing of the resulting organic phase of step (c) with, distilled water and further filtration in anhydrous magnesium sulfate or any other drier, which is unsolvable in water; (e) dehydration of the resulting solution of step (d) , resulting in an enriched-steroid fraction from ergostane derivative dry steroids; (f) isolation of the ergostane derivative dry steroids by means of medium pressure liquid chromatography (MPLC) and subsequent identification by spectroscopy.