ASSAY METHOD FOR IDENTIFYING INHIBITORS OF THE EXPRESSION OF THE ONCOPROTEINS E6 AND E7 OF THE HUMAN PAPILLOMA VIRUS (HPV), RECOMBINANT HOST CELL AND KIT FOR USE IN THE AFORESAID METHOD

摘要

The invention concerns an epithelial host cell stably transfected with a recombinant nucleic acid construct comprising the nucleic acid sequence of a reporter gene, for example luciferase, operatively linked with a second nucleic acid sequence comprising the enhancer elements and the promoter of the genes coding for the HPV proteins E6 and E7. The host cell of the invention is used as a reporter system in a high yield cell assay for the identification of molecules useful in the treatment of tumours induced by papilloma virus. The invention also concerns a kit for performing the assay.