The invention relates to a method for identifying dogs which are genetically normal, heterozygous for, or homozygous for the mutation primarily responsible for Collie eye anomaly (CEA). The method comprises the steps of obtaining a biological sample from a dog and testing DNA in the biological sample for the presence or absence of a 7.8 kilobase deletion within chromosome 37 in which the CEA mutation is located. No deletion is indicative of a normal dog. A deletion on one allele of chromosome 37 is indicative of a dog that is heterozygous for the CEA mutation. A deletion in both alleles of chromosome 37 are indicative of a dog that is homozygous for the CEA mutation. Also provided is a kit for identifying a dog as normal, heterozygous for, or homozygous for the CEA mutation.
申请公布号
EP1652937(A2)
申请公布日期
2006.05.03
申请号
EP20050256463
申请日期
2005.10.18
申请人
CORNELL RESEARCH FOUNDATION, INC.
发明人
ACLAND, GREGORY M.;KUKEKOVA, ANNA V.;AGUIRRE, GUSTAVO D.;OSTRANDER, ELAINE;AKEY, DAYNA;GOLDSTEIN, ORLY