| 摘要 |
1295992 Determination of glycerides BOEHRINGER MANNHEIM GmbH 31 Dec 1970 [2 Jan 1970] 61944/70 Heading B1X Protein-bound or protein-free mono, di- or tri-glycerides are determined using a completely enzymatic process which includes splitting the glycerides with a lipase obtained from Rhizopus arrhizus and determining the glycerol obtained in known manner. A test combination for use in the determination comprises phosphoenol pyruvate, ATP, lactate dehydrogenase, pyruvate kinase, glycerokinase, NADH and lipase obtained from a culture of Rhizopus arrhizus. In carrying out the test, (1) a serum sample may be mixed with solutions containing all the reagent components except the glycerokinase, the extinction measured at 366 mm, the glycerokinase added and after 10 minutes the extinction measured again; or (2) a buffer solution containing ATP may be mixed with a tablet containing NADH and phosphoenol pyruvate, the serum sample and a mixture of the enzymes (other than the lipase) added and the extinction measured, the lipase added and the extinction measured again. |
