| 摘要 |
Methods, systems and apparatus implement techniques for identifying modifications in polypeptides. A set of candidate sequences is identified that includes sequence information potentially corresponding to an unmodified variant of the polypeptide. Peptides derived from the polypeptide are sequenced to identify sequence tags. The sequence tags are compared with sequence information for the set of candidate sequences to identify a candidate sequence containing the sequence tags. For each such sequence tag, the difference between at least one subsequence mass of the corresponding peptide and at least one subsequence mass of the identified candidate sequence is calculated. The candidate sequences containing the sequence tags can be identified by searching a reduced database constructed based on the identified set of candidate sequences.
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