СПОСОБ ВЫЯВЛЕНИЯ ФОРМ ТРИХОМОНАД

摘要

FIELD: medicine, medicinal microbiology. ^ SUBSTANCE: for detection of trichomonas forms method involves blood seeding in Teras nutrient medium with addition of human or equine serum blood inactivated at temperature +56°C for 5-10 min and antibiotics in the dose 500 U/ml. Culture is incubated for 2 days at temperature +37°C followed by reseeding in similar medium but containing human or equine serum blood inactivated for 15-20 min and antibiotics in the dose 250 U/ml and incubated again at temperature +37°C for 2 days. Then reseeding is carried out in medium containing serum inactivated for 30 min and without addition of antibiotics and samples are incubated at temperature +37° for 2 days. Also, 1-2 additional reseeding are carried out in medium with completely inactivated human or equine serum blood and without addition of antibiotics. Based on active budding or shizogony the conclusion is done about the presence of live atypical forms of trichomonas and in case the absence of multiplication - about the presence of degenerative destructing forms. Using the method allows detecting atypical forms of trichomonas. ^ EFFECT: improved detecting method. ^ 1 tbl, 2 ex