发明名称 |
CELLULASE VARIANTS |
摘要 |
A method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:
a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG;
b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV;
c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5Å;
d. identifying surface-exposed amino acid residues of the enzyme;
e. identifying all charged or potentially charged amino acid residue positions of the enzyme;
f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided;
g. carrying out the substition, deletion or insertion by using conventional protein engineering techniques; and cellulase variants obtained by the method. |
申请公布号 |
EP0937138(B1) |
申请公布日期 |
2006.04.26 |
申请号 |
EP19970939989 |
申请日期 |
1997.09.17 |
申请人 |
NOVOZYMES A/S |
发明人 |
ANDERSEN, KIM, VILBOUR;SCHUELEIN, MARTIN;CHRISTIANSEN, LARS;DAMGAARD, BO;VON DER OSTEN, CLAUS |
分类号 |
C12N9/42;C12N15/09;C11D3/386;C12N1/15;C12N1/21;C12R1/645;C12S11/00;C12S99/00;D06L3/11;D06M16/00;D21B1/02;D21C5/02;D21H21/24 |
主分类号 |
C12N9/42 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|