发明名称 |
METHODS AND COMPOSITION FOR CONCENTRATING SECRETED RECOMBINANT PROTEINS |
摘要 |
Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein. |
申请公布号 |
CA2582808(A1) |
申请公布日期 |
2006.04.20 |
申请号 |
CA20052582808 |
申请日期 |
2005.10.03 |
申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
TARON, CHRISTOPHER H.;COLUSSI, PAUL A. |
分类号 |
C12N15/81;C12N9/24;C12P21/02 |
主分类号 |
C12N15/81 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|