发明名称 |
SIMULTANEOUS SEQUENCE ANALYSIS OF AMINO- AND CARBOXY- TERMINI |
摘要 |
The present invention relates to a new method for identifying polypeptides by deducing the amino acid sequence of the carboxy and amino termini by a mass spectrometer analysis. The method comprises the steps of dissociating highly charged peptide precursor ions (e.g., z>4) using electron transfer dissociation inducing anions followed by removal of those reagents and introduction of a second, proton transfer inducing anion type. The second PTR reaction duration is adjusted to convert the ETD products to primarily the +1 charge-state to reduce the highly charged c and z-type fragments, producing an m/z spectrum containing a series of c and z-type fragment ions that are easily interpreted to reveal the sequence of the amino and carboxy terminus, respectively. |
申请公布号 |
CA2582578(A1) |
申请公布日期 |
2006.04.20 |
申请号 |
CA20052582578 |
申请日期 |
2005.10.07 |
申请人 |
UNIVERSITY OF VIRGINIA PATENT FOUNDATION |
发明人 |
HUNT, DONALD F.;SYKA, JOHN EDWARD PHILIP;COON, JOSHUA J. |
分类号 |
G01N33/68 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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