TARGETED TRANS-SPLICING OF HIGHLY ABUNDANT TRANSCRIPTS FOR IN VIVO PRODUCTION OF RECOMBINANT PROTEINS
摘要
The present invention provides methods and compositions for generating novel nucleic acid molecules through RNA trans-splicing that target a highly expressed pre-mRNA and contain the coding sequence of a protein or polypeptide of interest. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with the target precursor messenger RNA molecule (target pre-mRNA) that is abundantly expressed, and mediate a trans-splicing reaction resulting in the generation of novel chimeric RNA molecule (chimeric RNA) capable of encoding a protein or polypeptide of interest. The invention provides for the <i
申请公布号
WO2006042232(A2)
申请公布日期
2006.04.20
申请号
WO2005US36424
申请日期
2005.10.07
申请人
INTRONN, INC.;MCGARRITY, GERARD, J.;GARCIA-BLANCO, MARIANO, A.