Modulation of interaction between Nod1 and Gram-negative bacteria by Nod1 response to peptidoglycan delivered by the Helicobacter pylori cag pathogenicity island (cagPAI)

摘要

A method for identifying a compound that modulates the interaction between Nod1 and a Gram-negative bacteria comprising contacting a Nod1 expressing cell with a cagPAI-positive H. pylori in the presence of a compound, contacting a Nod1 expressing cell with a cagPAI-positive H. pylori in the absence of said compound, and detecting the activation of a pro-inflammatory factor and/or the production of a pro-inflammatory cytokine or chemokine, wherein altered activation and/or production indicates that said compound modulates the interaction between Nod1 and the Gram-negative bacteria. The method is useful for identifying compounds for regulating the signaling cascade involving Nod1 against Gram-negative bacteria that cause mucosal inflammation in animal hosts.