Substrates for hybridization and method of using the same

摘要

A hybridization substrate having on a substrate surface thereof a branching nucleic acid strand comprising a principal strand of nucleic acid a portion of which is attached to the surface of the substrate and a portion of which is single-stranded, and a strand of single-stranded nucleic acid for probe that is partially hybridized to a portion of the single-stranded portion of the principal nucleic acid. A hybridization substrate having on a substrate surface thereof a branching nucleic acid strand comprising a principal strand of nucleic acid a portion of which is attached to the surface of the substrate and a portion of which is single-stranded; an accessory strand of nucleic acid that is single-stranded and partially hybridized to a portion of the single-stranded portion of the principal strand of nucleic acid; a strand of single-stranded nucleic acid for probe partially hybridized to a portion of the single-stranded portion of the principal strand of nucleic acid; and/or a strand of single-stranded nucleic acid for probe partially hybridized to a portion of the accessory strand of nucleic acid. A method comprising bringing target nucleic acid into contact with a surface upon which has been immobilized the branching nucleic acid strand of the above-mentioned substrate to test the complementarity of the target nucleic acid with the probe area of the branching nucleic acid strand. Provided are a hybridization substrate upon the surface of which DNA strands are immobilized and a complementarity test method employing this substrate.