METHOD FOR DETECTING GENE POLYMORPHISM OF APOLIPOPROTEIN E, METHOD FOR DETERMINING GENOTYPE OF GENE POLYMORPHISM OF APOLIPOPROTEIN E

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting a gene polymorphism of an apolipoprotein E, improved with accuracy and reliability, a method for determining the genotype of the gene polymorphism of the apolipoprotein E and their reagents. SOLUTION: This method for detecting the gene polymorphism of the apolipoprotein E is characterized by comprising a step of amplifying a DNA by using a primer pair specific to the apolipoprotein E gene; a step of cutting off the amplified DNA by using a first restriction enzyme containing a single base polymorphic site of a codon 112 of the apolipoprotein E gene in its recognition sequence and a second restriction enzyme containing the single base polymorphic site of codon 158 of the apolipoprotein E gene in its recognition sequence; a step of separating the cut DNA fragments; and a step of detecting the separated DNA fragments, and using an upstream side primer among the primer pairs, having at least one base mismatch sequence introducing the recognition sequence of the first restriction enzyme to more upstream side than the recognition sequence of the first restriction enzyme containing the single base polymorphic site of the codon 112. COPYRIGHT: (C)2006,JPO&NCIPI