ASSAY FOR DETECTING AND QUANTIFYING H1V-1

摘要

A reaction mixture for amplifying either HIV-1 M group nucleic acids or HIV-1 O group nucleic acids is provided. The reaction mixture comprises (a) a first amplification primer comprising a first primer target-hybridizing sequence that independently hybridizes to a first strand of HIV-1 M group nucleic acids and a first strand of HIV-1 O group nucleic acids; (b) a second amplification primer comprising a second primer target-hybridizing sequence that hybridizes to an enzymatic extension product of said first amplification primer using as a template either said first strand of HIV-1 M group nucleic acids or said first strand of HIV-1 O group nucleic acids; and (c) a molecular torch hybridization probe that hybridizes to an amplicon synthesized by the combined activity of said first amplification primer and said second amplification primer, wherein neither said first primer target-hybridizing sequence nor said second primer target-hybridizing sequence is fully complementary to said HIV-1 M group or HIV-1 O group nucleic acids or the complement thereof, and wherein said HIV-1 M group nucleic acids and HIV-1 O group nucleic acids amplify in the reaction mixture with substantially equal efficiency.