ASSAY FOR CYTOCHROME P450 ISOFORMS 3A4 AND 3A5

摘要

A rapid and sensitive radiometric assay for assessing the activity of cytochrome P-450 (CYP) 3A4/5 and the potential of an analyte to inhibit CYP3A4/5 activity or induce CYP3A4/5 expression is described. All the steps of the assay, including incubations, product separation, and radioactivity counting are preferably performed in a multiwell format, which can be automated.