发明名称 |
Method for obtaining anti-idiotype antibodies |
摘要 |
A method for identifying molecules which mimic an idiotype of an autoimmune disease-associated auto-antibody (autoantibodies). The method comprises the following steps: (a) purifying autoantibodies from sera of one or more patients afflicted with the autoimmune disease; (b) binding the autoantibodies to a solid phase to form an affinity matrix; (c) contacting pooled plasma or B cells comprising immunoglobulins with the affinity matrix followed by removal of unbound plasma components; (d) eluting bound immunoglobulins, being anti-Idiotypic antibodies (anti-Id) to autoantibodies, from the matrix; (e) providing a molecular library comprising a plurality of molecule members; and (e) contacting the anti-Id with the molecular library and isolating those bound molecules which are bound by the anti-Id, the bound molecules being molecules which mimic an idiotype of autoantibodies. Also disclosed are such molecules.
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申请公布号 |
US2006078943(A1) |
申请公布日期 |
2006.04.13 |
申请号 |
US20030515499 |
申请日期 |
2003.05.22 |
申请人 |
OMRIX BIOPHARMACEUTICALS INC. |
发明人 |
NUR ISRAEL;SHOENFELD YEHUDA |
分类号 |
C40B40/10;A61K38/06;A61K38/12;A61K39/00;C07K7/06;C07K16/06;C07K16/18;C07K16/42;C40B30/04;G01N33/564;G01N33/68 |
主分类号 |
C40B40/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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