A system has been constructed that recapitulate the features of a capillary bed through normal human tissue. The system facilitates perfusion of three-dimensional (3D) cell monocultures and heterotypic cell co-cultures at the length scale of the capillary bed. A major feature is that the system can be utilized within a "multiwell plate" format amenable to highthroughput assays compatible with the type of robotics commonly used in pharmaceutical development. The system provides a means to conduct assays for toxicology and metabolism and as a model for human diseases such as hepatic diseases, including hepatitis, exposure-related pathologies, and cancer. Cancer applications include primary liver cancer as well as metastases. The system can also be used as a means of testing gene therapy approaches for treating disease and inborn genetic defects.
申请公布号
WO2005123950(A3)
申请公布日期
2006.04.13
申请号
WO2005US17594
申请日期
2005.05.19
申请人
MASSACHUSETTS INSTITUTE OF TECHNOLOGY;UNIVERSITY OF PITTSBURGH;DOMANSKY, KAREL;GRIFFITH, LINDA, G.;TANNENBAUM, STEVEN, R.;WELLS, ALAN
发明人
DOMANSKY, KAREL;GRIFFITH, LINDA, G.;TANNENBAUM, STEVEN, R.;WELLS, ALAN