| 发明名称 | 红麻微卫星DNA标记 | ||
| 摘要 | 本发明涉及红麻的DNA分子遗传标记。本发明分离克隆了5个多态性丰富的红麻微卫星DNA标记的DNA序列和扩增该5个红麻微卫星位点的引物序列。本发明的红麻微卫星DNA标记用于红麻品种分类、遗传关系鉴定和标记辅助育种，重复性好，是一种可靠有效的分子标记。 | ||
| 申请公布号 | CN1757731A | 申请公布日期 | 2006.04.12 |
| 申请号 | CN200510031930.4 | 申请日期 | 2005.07.28 |
| 申请人 | 湖南师范大学 | 发明人 | 周建林;揭雨成;蒋彦波;张健 |
| 分类号 | C12N15/29(2006.01);C12Q1/68(2006.01);C07H21/04(2006.01) | 主分类号 | C12N15/29(2006.01) |
| 代理机构 | 长沙永星专利事务所 | 代理人 | 周咏;林毓俊 |
| 主权项 | 1、适于红麻品种分类、遗传关系鉴定和辅助育种的微卫星DNA标记，其特征在于，所述的编号分别为HCM30、HCM31、HCM32、HCM33、HCM34的五个微卫星DNA标记的核苷酸序列如下：HCM30：aaaggaaatt caagatgcaa gaggtgaagg aagtgattat gtttggtatt tgcaacaaca 60aatgtctagg aacattgatt tatcgtagtt tcaatgaaaa ttgttccttc gtattttgga 120aatttttttt atttctttta agagataata ggaaagatag tgtattagcg tggaaatgtg 180aattcatata tgttgctcaa ctcaaagtac tagtttttgc agaggccaag tgctaatttt 240gggcattttc caagggcaca aagaggcacg tgcgaatctg ccacatgtac tgacccaccc 300ctctctctct ctccctctct ctctctctct ctctctctct ctctctcaaa tttttctcta 360agcagacatg gccaacaatt cttcttcatc agccaaatac acaatggtgg cagccaagaa 420ccgctgggaa gagcaaggat tctatctgga tgacagccaa gttaaatacg gcctggagcc 480gatcattcac aaaaggctgc acgaactcag ctagttccgc cttgcaagca gccagccagg 540gccaact 547HCM31：aaaaggaaat tcaagatgca agaggtgaag aaagtgatta tgtttggtat ttgcaacaac 60aaatgtctag gaacattgat ttatcgtagt ttcaatgaaa attgttcctt cgtattttgg 120aaattttttt tatttctttt aagagataat aggaaagata gtgtattagc gtggaaatgt 180gaattcatat atgttgctca actcaaagta ctagtttttg cagaggccaa gtgctaattt 240tgggcatttt ccaagggcac aaagaggcac gtgcgaatct gccacatgta ctgacccacc 300cctctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctctaaagc 360tggggttttt gcaaagaatt tgccgagaca tggccaaatg agtaatctgg ccatcaattt 420tcttaacatt gaattgtgtc tagctggatt gaagaacgta gtttctggtt ctttctatta 480gaaccttcct ttgtaatact tggggattga actcttccaa gagaaccaag acccaagttt 540gatttgattg tgttagatta cagtgtattt taggtgaaag ctgtatctgc aagagtttag 600ccactttctt ggatattgga atcaattact taactaaaat aaaaacaaaa atgtgcatag 660tgttttcatt ctttagtcgc cgatgtataa taatagttta agtttattga ctcttctgat 720tctaggcttt ctagatttga cttgtctgtt aaagactgtg gttcttcttt ttctggtaaa 780ttgatattgg atgtgcctac tcgatggaac tctacttatt 820HCM32：acagggaaaa atgaaggcat aatccgatca tccgagtttt ggccgaagaa actccgttga 60aaccccacct tgtgttgtgc atcagccaag tatcagattt actaccgttt gagcggagat 120cgcgcccgga gcacctagaa acttggaaaa aattgacttt aatttcgaat tcatgggtat 180accaatcggg tttgtgggtc tctcccgtag taagtaactc catctctctc tctctcttcc 240gttcatctct ctctctctct ctctctctct ctctctctct ctctctctct ctctctctct 300ctctcttttt gtttcttcca ttttctttct ttccaaaaac tgaaactttc ttggcattcg 360ttacgattta taattagccc ccacccctaa aaattttaca taaaaaagac agccagatcc 420ccaccattga aatgatgctt tgctttgtag cattcatcat caccgtccaa tccaatgcct 480gcagcaatgg atgacccata atccggtaca agcccgagca ttttctgtaa gccccccccc 540cccaaaaaaa aaaaaaaacc aaccatgctc gcctttgttg aaattaaaga agttcgacgg 600tgaagaaatc atttaagaaa tcagtgaatt cacctaatga ggtgaatttg atcaatgaag 660gat 663HCM33：acaactcacc actgtgatgg accaattgac tcatcgtcaa cagaatgatt gatcggctca 60ctttacaacg atcggtcggc tccttgcaat gaccgattgg tccagttggc tcgtcaccga 120catgatgacg agttggttct ccttctcaac gttagattgg ctcgctttct ggccaccaac 180ataatgatta aataaatttt attctctctc aactcatacc aataattact tcacaaatat 240catttttcta cttcttttat cttttctctc taggctctct ctttcttccg atcttctttc 300ttttctctct ctctctctct ctctctctct ctctctctct ctctttgtcc ttttctccat 360ctttgtctcc tagtgcgccc agattttgtc cagaatgcct caacatccac atcatcccac 420agatccttgt agaaatgcta cagcttcttc tcctt 455HCM34：attttttgcg tccatgtttg cttaagcagt caccacctat ttttgctcat tctttgtttt 60tcttctctct cactttctca cttcgctttc ctatctttct cttcgttttg agatcttttg 120gttcatcctt tgtttagttg ttgtggatgt ttctccctga cgtttttggc tctgtgcggg 180ccctcttaag tgaacgagac tatagattgg tgatagagaa gagtttttaa ccagttttca 240tcctcctttt tctgatgacg aggcacttca gtgtcgtagc ctacatcatt tcttaagctt 300ttgtgatgcc tttcatctaa ccgcaatgct tgatgttagt ctctctccac atctccttca 360aactctcgat acttttgtat gcatcagatt tgacacacaa cttgatgagt ggacgctagt 420tctctctctc tctctctctc tctctctctc tctctctctc tcatacaata gtgtatgaca 480taactatggt ttttggaaac aatagcagaa ttcaaactaa cgctatacaa gaggaggaaa 540aacctgccta ttggtttact ttccagtttc aactagagat tcccattaat gtaagggaag 600aagtggaagt gcattgttaa gtgcacttat tgttgtcgag ttaaaacaga tgagggtttt 660gaactgcctg cctgtgatcc tagtttgtat aaggttatgt ttgcatagcc aacttgagat 720atagtt 726 | ||
| 地址 | 410081湖南省长沙市河西二里半177号 | ||