Method of counting microorganisms or cells

摘要

A method of counting microorganisms or cells in a sample by labeling the microorganisms or cells with a fluorescent labeling reagent, which comprises contacting the sample containing the microorganisms or cells with an adhesive sheet having an adhesive layer laminated on at least one surface of a substrate to capture the sample; before fluorescent-labeling the microorganisms or cells, obtaining a first fluorescent image of the sample; after fluorescent-labeling the microorganisms or the cells, obtaining a second fluorescent image of the sample, and then determining the difference in the number of luminous points between the first and second images, or determining a differential image between the first and second images and determining the number of luminous points in this differential image, or determining the number of luminous points among the luminous points in the second image located outside non-sensitive regions attached to the individual luminous points in the first image.