The present invention provides fluorescent cyanine dye-based nucleotide analogues, in which the cyanine dye is coupled to the -phosphate group of a nucleoside triphosphate. Preferred embodiments of the invention are directed to fluorescent cyanine dye-based GTP analogues which may be employed in an homogeneous FRET-based assay to measure the binding of guanine nucleotides to GPCR polypeptides, or alternatively, to measure the effect of an exogenous ligand on GPCR protein binding.
申请公布号
WO2006035207(A2)
申请公布日期
2006.04.06
申请号
WO2005GB03685
申请日期
2005.09.27
申请人
AMERSHAM BIOSCIENCES UK LIMITED;BRIGGS, MARK, SAMUEL, JONATHAN;SMITH, TONY;SANTOS, ALBERT, FRANCIS
发明人
BRIGGS, MARK, SAMUEL, JONATHAN;SMITH, TONY;SANTOS, ALBERT, FRANCIS