RECOMBINANT DNA VIRUSES COMPRISING A DNA THAT IS COMPLEMENTARY TO AN RNA VIRUS REPLICON, AND APPLICATIONS THEREOF

摘要

The invention relates to recombinant DNA viruses comprising a DNA that is complementary to an RNA virus replicon, and to the applications thereof. According to the invention, the genome of the recombinant DNA virus comprises all or part of the genome of a DNA virus and a sequence of the cDNA of a replicon derived from an RNA virus that contains a nucleotide sequence encoding an exogenous protein or a fragment of same and, optionally, a nucleotide sequence encoding the structural proteins of the aforementioned RNA virus or proteins encapsulating said replicon. The inventive recombinant DNA viruses can be used to produce single-cycle chimeric viral particles containing a replicon derived from an RNA virus, in order to produce exogenous proteins of interest or fragments of same, as well as for therapeutic applications such as in the formulation of vaccines.