摘要 |
<p>Use of fibroblasts to produce differentiated animal cells, is new. Independent claims are also included for: (1) producing adipocytes by culturing fibroblasts first in a medium supplemented with dexamethasone, indomethacin, transforming growth factor beta (TGFbeta ), insulin, 3-isobutyl-1-methylxanthine, transferrin, fetal calf serum, penicillin and streptomycin and then in a medium supplemented with TGFbeta 3, insulin, fetal calf serum, penicillin and streptomycin, the media being alternated 2-5 times; (2) producing chondrocytes by culturing fibroblasts in a medium supplemented with dexamethasone, sodium pyruvate, ascorbic acid 2-phosphate, proline, insulin, transferrin, selenous acid, linoleic acid, bovine serum albumin, TGFbeta 3, penicillin and streptomycin; (3) producing osteocytes by culturing fibroblasts first for several hours in a medium supplemented with insulin, transferrin, selenous acid, linoleic acid, bovine serum albumin, TGFbeta 3, penicillin and streptomycin and then for several weeks in a medium supplemented with insulin, transferrin, selenous acid, linoleic acid, bovine serum albumin, dexamethasone, ascorbic acid 2-phosphate, human fibronectin and glycerophosphate. ACTIVITY : Vulnerary. MECHANISM OF ACTION : Cell and tissue replacement.</p> |