METHOD OF PRODUCING ALDONIC ACIDS AND ALDOSE DEHYDROGENASE

摘要

Aldonic acids are efficiently produced by coupling a reaction of oxidizing aldose by aldose dehydrogenase using nicotinamide adenine dinucleotide (NAD+) as a coenzyme with a NAD+ regeneration system using NADH oxidase. Also, it is intended to provide a recombinant microorganism in which NADH oxidase and aldonic acid dehydrogenase are expressed in a single host; an aldose dehydrogenase using NAD as a coenzyme; and a composition containing NADH oxidase. Thus, aldonic acids, which have been widely used in food additives, feed additives, medicinal additives and so on, can be efficiently produced by a convenient method.