PREPARATION AND USE OF SINGLE-STRANDED TRANSCRIPTION SUBSTRATES FOR SYNTHESIS OF TRANSCRIPTION PRODUCTS CORRESPONDING TO TARGET SEQUENCES

摘要

The present invention provides methods, compositions and kits for using an RNA polymerase for making transcription products corresponding to a target sequence by obtaining single-stranded DNA transcription substrates that comprise a single-stranded promoter that is operably joined to a single-stranded target sequence. The invention has broad applicability for research, diagnostic and therapeutic applications, such as preparing cDNA corresponding to full-length mRNA, making sense or anti-sense probes, detecting gene- or organism-specific sequences, cloning, cell signaling or making RNA for use in RNAi. Fig.1 shows bacteriophage N4 vRNAP promoters that are characterized by conserved sequences and a 5 base pair stem, 3 base loop hairpin structure.