发明名称 |
GLYCOSYLATED HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) ISOFORM |
摘要 |
Disclosed are human G-CSF isoforms having a modified amino acid sequence containing a glycosylation sequence Asn-X-Ser/Thr (N-X-S/T) at one or more specific amino acid positions according to the present invention, genes encoding the human G-CSF isoforms, and expression vectors carrying the genes, eukaryotic cells transformed or transfected with the expression vectors. Also, the present invention discloses a method of preparing a glycosylated human G-CSF isoform, comprising the steps of culturing the transformant or transfectant and isolating a glycosylated human G-CSF isoform from the culture supernatant or cell lysates, a human G-CSF isoform prepared by the method, and a pharmaceutical composition comprising the human G-CSF isoform. |
申请公布号 |
EP1551870(A4) |
申请公布日期 |
2006.03.29 |
申请号 |
EP20030748751 |
申请日期 |
2003.08.29 |
申请人 |
CJ CORP. |
发明人 |
LEE, EUN-JUNG;KIM, HYUN-SEOK;CHUNG, JONG-SANG;KIM, KI-WAN;KIM, YEON-HYANG;LEE, HYUNE-SOO;KOH, HYUNG-KON;OH, MYUNG-SUK |
分类号 |
C07K14/47;A61K38/00;C07K14/535 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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