发明名称 |
CHEMILUMINESCENCE REDOX ASSAY TO QUANTIFY ANALYTES IN BIOLOGICAL SAMPLES |
摘要 |
<p>A method for determining concentration of an analyte in a biological sample comprising the steps of: (a) combining the biological sample, at least one oxidizing enzyme for the analyte of interest, nicotinamide adenine dinucleotide (hereinafter NAD+), and a chemiluminescent label to form a reaction mixture; (b) allowing the analyte to undergo an oxidation-reduction reaction and NAD+ to be converted to the reduced form of nicotinamide adenine dinucleotide (hereinafter NADH) and further allowing the chemiluminescent label to react with NADH; and (c) determining the concentration of the analyte of interest in the biological sample by correlating the quantity of light emitted with the concentration of NADH.</p> |
申请公布号 |
EP1192467(B1) |
申请公布日期 |
2006.03.29 |
申请号 |
EP20000942905 |
申请日期 |
2000.06.16 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
VIDAKOVIC, MOMCILO, S.;LEAHY, DAVID, C.;MASSIE, DONNA, M. |
分类号 |
G01N33/533;G01N33/58;C12Q1/26;C12Q1/32;G01N21/78;G01N33/98 |
主分类号 |
G01N33/533 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|