发明名称 |
Cloud-point extraction of enzymes and polypeptides from a fermentation broth using a non-ionic surfactant |
摘要 |
A method of extracting a polypeptide of interest from a fermentation broth comprising: i) adjusting the pH close to pI of the polypeptide of interest; ii) adding a non-ionic surfactant with a hydrophile-lipophile balance (HLB) of 12 or lower; iii) cooling the mixture for solubilization and incubating at above cloud point for extraction; iv) phase separating at below cloud point to obtain liquid-liquid-solid fractions; and v) recovering the surfactant-rich top phase containing the polypeptide of interest.
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申请公布号 |
US7019120(B2) |
申请公布日期 |
2006.03.28 |
申请号 |
US20030734431 |
申请日期 |
2003.12.12 |
申请人 |
NOVOZYMES A/S |
发明人 |
IYER PRASHANT;RANE KISHORE;WENGER KEVIN S.;AZZABI FAHD |
分类号 |
C07K1/14;C07K1/36;C12N9/00 |
主分类号 |
C07K1/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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