Propargylethoxyamino nucleotides

摘要

Propargylethoxyamino nucleosides are disclosed having the structure wherein R₁ and R₂ are -H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W₁ is -H or -OH; W₂ is -OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position; and W₃ is -PO₄, -P₂O₇, -P₃O₁₀, phosphate analog, or -OH. Additionaly, a primer extension method is provided employing the above propargylethoxyamino nucleosides.