METHOD OF PURIFYING NUCLEIC ACID USING NONWOVEN FABRIC AND DETECTION METHOD

摘要

A method of separating and purifying a nucleic acid from a sample containing cells such as blood or a liquid culture medium. In this method, a cell extract obtained by disrupting cell is adsorbed by a filter made of a nonwoven fabric and, after washing the filter, the nucleic acid is eluted therefrom. The nucleic acid may be eluted under alkaline conditions (pH 12 or higher). It is also possible to elute the nucleic acid by treating the nucleic acid adsorbed by the filer with active oxygen or using a surfactant. Nucleic acids separated and purified by the above method are usable in nucleic acid amplification or base sequence analysis techniques.