| 摘要 |
<p>The present invention is directed to methods and compositions for performing proximity assays in samples of fixed specimens. In particular, the assays are useful for the detection of multicomponent complexes of biological molecules such as proteins, peptides, hormones, DNA, RNA and the like. In one embodiment, the method comprises providing a signal generating system comprised of a first unit capable of enzymatically producing a transmitter and having a first binding moiety, and a second unit dependent on the transmitter for producing a signal molecule and having a second binding moiety, combining under binding conditions the sample and the signal generating system such that the first and second binding moieties bind to their respective binding sites on different components of the complex such that the second unit is within the effective proximity of the transmitter produced by the first unit so that signal molecules are produced, and analyzing for the presence of signal molecules, whereby the multicomponent complex is detected. Also provided are compositions that have an enzymatic unit for producing a transmitter that then non-enzymatically generates a signal at the second unit.</p> |
