Standard micro-component for calibrating or standardizing fluorescence measuring instruments and biochip comprising same

摘要

The invention concerns a standard micro-component ( 4 ) for calibrating or standardizing fluorescence measuring instruments, comprising a substrate ( 1 ) whereon is arranged at least one thin film ( 2 ). The thin film ( 2 ) includes fluorescent components. At least one first zone ( 3 ) of null thickness is formed in the thin film ( 2 ), thereby exposing the substrate ( 1 ). The thin film ( 2 ) comprises at least one exposed zone ( 2 a), such that first and second fluorescence levels are respectively defined in the non exposed part and in the exposed part ( 2 a) of the thin film ( 2 ). The second fluorescence level is lower than the first fluorescence level. The standard micro-component ( 4 ) can also include a plurality of stacked thin films so as to define a plurality of fluorescence levels.